A Multi-Disciplinary Framework for Decoding S1PR1-Selective Agonism

Sphingosine-1-phosphate (S1P), a key metabolite of sphingolipids, plays crucial roles in a wide range of physiological and pathological processes. S1P primarily exerts its functions by binding to G protein-coupled sphingosine-1-phosphate receptors (S1PRs), which include five subtypes (S1PR1–5), thereby activating these receptors and their downstream signaling pathways. Understanding the molecular determinants that govern agonist selectivity among different S1PR subtypes is vital for the rational and precise development of targeted therapeutic agents. Here, four cryo-electron microscopy structures of agonist-bound S1PR1-Gi complexes are reported. Through an integrated approach combining structural analysis, molecular dynamics simulations, and pharmacological assays, the molecular basis for the selectivity of CYM5442, HY-X-1011, Ponesimod, and SAR247799 toward S1PR1 over S1PR3 and S1PR5 is uncovered. Specifically, the selectivity arises from a combination of non-conserved residues within the ligand-binding pocket and at the Gi-protein interface, distinct curved agonist-binding modes oriented toward transmembrane helices 5-7 that cause steric clashes with S1PR3, and the presence of branched moieties at the lower part of three agonists. These features collectively enhance agonist potency and efficacy for S1PR1 while reducing activity at S1PR3 and S1PR5. These findings establish a structural framework for the rational design of next-generation S1PR1 highly selective agonists with improved therapeutic potential.