Virotrap Reveals Salmonella SopB as A Ubiquitinated Cargo for Host ESCRT-0

The pathogenic bacterium Salmonella survives and replicates in host cells within a Salmonella -containing vacuole (SCV). To build this niche, Salmonella uses secreted effectors, such as SopB, a phosphoinositide phosphotransferase that modifies host phospholipid fluxes at the plasma membrane and SCV. These lipid modifications impact host protein recruitment and activity, implicating SopB in bacterial internalization, SCV biogenesis, and inflammatory signaling. Yet, interactions of SopB with host proteins have remained ill-defined. Here, we employ the unique Virotrap mass spectrometry-based technology to identify a new set of SopB-associated host proteins. We demonstrate the direct interaction of SopB with the ubiquitin-binding domains of the ESCRT-0 subunit HGS, and we show that SopB promotes ESCRT-0 recruitment at the SCV where they colocalize. As the ESCRT machinery plays a central role in cargo sorting and membrane remodeling, we propose a new SopB-dependent mechanism by which Salmonella controls host membrane dynamics.