groovDB in 2025: A community-editable database of small molecule biosensors

The groovDB database (https://groov.bio) was launched in 2022 with the goal of organizing information on prokaryotic ligand-inducible transcription factors (TFs). This class of proteins is important in fundamental areas of microbiology research and for biotechnological applications that develop biosensors for diagnostics, enzyme screening, and real-time metabolite tracking. Uniquely, groovDB contains stringently curated, literature-referenced data on both TF:DNA and TF:ligand interactions. Here, we describe a major update to groovDB, making the database community-editable and adding several advanced features. Users can now add new TF entries and update existing entries using a simple online form. New user interface elements allow viewing of interactive TF protein structures and DNA-binding motifs. Updated query methods enable text-based search, chemical similarity search, and attribute-filtering searches. A new data architecture improves web interface responsiveness by over five-fold. Finally, the number of TF entries has more than doubled and all source code is now open-access.