Facilitating analysis of open neurophysiology data on the DANDI Archive using large language model tools

The DANDI Archive is a key resource for sharing open neurophysiology data, hosting over 400 datasets in the Neurodata Without Borders (NWB) format. While these datasets hold tremendous potential for reanalysis and discovery, many researchers face barriers to reuse, including unfamiliarity with access methods and difficulty identifying relevant content. Here we introduce an AI-powered, agentic chat assistant and a notebook generation pipeline. The chat assistant serves as an interactive tool for exploring DANDI datasets. It leverages large language models (LLMs) and integrates with agentic tools to guide users through data access, visualization, and preliminary analysis. The notebook generator analyzes dataset structure with minimal human input, executing inspection scripts and generating visualizations. It then produces an instructional Python notebook tailored to the dataset. We applied this system to 12 recent datasets. Review by neurophysiology data specialists found the generated notebooks to be generally accurate and well-structured, with most notebooks rated as “very helpful.” This work demonstrates how AI can support FAIR principles by lowering barriers to data reuse and engagement.