Impaired systemic antibody response against gut microbiota pathobionts in critical illness and susceptibility to nosocomial infections

Critically ill patients in intensive care units (ICUs) experience high rates of nosocomial infections, commonly caused by translocation and dissemination of pathogenic microorganisms that colonize the intestinal tract (pathobionts). Multiple immune barriers protect the host against commensal and pathogenic colonizers, including a repertoire of circulating anti-commensal antibodies. The integrity of this systemic antibody-mediated defense system, its relationship with gut microbiota dysbiosis, and its impact on nosocomial infections in the ICU have not been explored. We observed markedly impaired plasma IgM and IgG reactivity against intestinal pathobionts such as Escherichia coli , Klebsiella pneumoniae , and Enterococcus faecalis in ICU patients compared to healthy volunteers. Reduced gut pathobiont antibody responses in ICU patients was associated with B cell lymphopenia, and patients with gut microbiota dysbiosis had reduced levels of natural antibody producing B1-like B cells. Reduced IgG against gut Gram-negative pathobionts was associated with an increased risk of nosocomial infection or death. These findings indicate that the systemic antibody barrier against microbiota pathobionts is compromised in critical illness and associated with increased risk of nosocomial infections, identifying a potential role for therapeutic antibody supplementation to prevent infections in the ICU.