Uncharacterized Acetilactobacillus -like genera represent the most abundant microorganisms in honeys produced by native stingless bee

Meliponiculture is deeply rooted in Mexico, especially in the Yucat&aacuten Peninsula and Veracruz, where it serves as both an economic activity and a cultural tradition. While Lactobacillus species are known to be abundant in the microbiomes of honeybees and their honey, most studies have focused on Apis mellifera , leaving stingless bee honey largely unexplored. Here, we report the first shotgun metagenomic analysis of honey from two extensively managed stingless bee species: Melipona beecheii and Scaptotrigona mexicana . From these samples, we reconstructed 25 metagenome-assembled genomes (MAG), 15 of which showed no match any known species, with average nucleotide identity (ANI) around 79 against its closest relative Acetilactobacillus jinshanensis , suggesting they represent novel species. Clustering revealed four distinct groups (intragroup ANI >99; intergroup ANI <80), possibly representing four new species across two new genera. Metapangenomic analysis further confirmed that reads did not align to existing Acetilactobacillus references, indicating that Acetilactobacillus -like microbes are novel and abundant. Given that Acetilactobacillus is underrepresented genomically and exhibits a long evolutionary branch within Lactobacillaceae , our findings highlight the potential importance of these microbes. Stingless bee honey has been attributed to antioxidant and antibacterial properties, which may be influenced by its microbial content. Acetilactobacillus -like bacteria may play key roles in shaping these properties in acidic food microbiomes. Our study provides foundational insights into the unique microbial diversity of stingless bee honey and its potential contributions to honey′s chemical and medicinal characteristics.