MAPseq2: a sensitive and cost-effective barcoded connectomics method
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a format solvable by DNA sequencing. Here we present MAPseq2, a user-friendly protocol with up to ∼10-fold increased sensitivity and ∼10-fold decreased cost compared to the current state of the art. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRlCseq, and ConnectlD, all improvements in MAPseq2 directly transfer to these technologies.
