Click-qPCR: an ultra-simple tool for interactive qPCR data analysis

Real-time quantitative PCR (qPCR) is a pivotal technique for gene expression and DNA copy number variation analysis. However, the limited availability of easy-to-use software tools remains a significant challenge for experimental biologists without programming expertise. To address this issue, we developed Click-qPCR, a user-friendly, web-based Shiny application that requires no local installation. Click-qPCR streamlines ΔCt and ΔΔCt calculations using user-uploaded CSV data files. The application allows users to dynamically select genes and groups, and perform Welch’s t-tests for group comparisons. Results are immediately visualized as interactive bar plots (mean ± SD with individual data points) and can be downloaded as publication-quality images, along with statistical summaries. Click-qPCR enables researchers to efficiently process, interpret, and visualize their qPCR data regardless of programming skills, thereby accelerating data processing and improving the accessibility of routine analysis.