Charting the Circulating Proteome in ME/CFS: Cross System Profiling and Mechanistic insights
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Abstract
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating condition often triggered by infections. The underlying mechanism remains poorly understood, and diagnostic markers and effective treatments are presently lacking. We performed aptamer-based serum proteomics in 54 ME/CFS patients and 27 healthy controls and identified 1823 of 7326 aptamers reporting differences between the groups (845 after false discovery rate (FDR) correction). Distinct patterns of tissue- and process-specific changes were seen. There was a broad increase in secreted proteins, while intracellular proteins, e.g. from skeletal muscle, particularly showed reduction. Immune cell-specific signatures indicated immune reprogramming, including a distinct reduction in neutrophil-associated proteins. Focused secretome analysis supported intensified regulatory interactions related to immune activity, inflammation, vasculature, and metabolism. Validation of measurements using antibody-based methods confirmed findings for a selection of proteins. The uncovered serum proteome patterns in ME/CFS patients help clarify a multifaceted pathophysiology and offer a foundation for future therapy and biomarker discovery efforts.
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Reviewer(s): B Lidbury (The Australian National University) | 📗📗📗📗◻️
S Elahi (University of Alberta) | 📒📒📒◻️◻️
A Rosén & S Sayyab (Linköping University) | 📗📗📗📗◻️ -
Anders Rosén, Shumaila Sayyab
Review 3: "Charting the Circulating Proteome in ME/CFS: Cross System Profiling and Mechanistic insights"
Reviewers remarked that the study described in the preprint was an ambitious, well-planned and comprehensive profiling of serum proteins that have altered expression in patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).
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Brett Lidbury
Review 1: "Charting the Circulating Proteome in ME/CFS: Cross System Profiling and Mechanistic insights"
Reviewers remarked that the study described in the preprint was an ambitious, well-planned and comprehensive profiling of serum proteins that have altered expression in patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).
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Shokrollah Elahi
Review 2: "Charting the Circulating Proteome in ME/CFS: Cross System Profiling and Mechanistic insights"
Reviewers remarked that the study described in the preprint was an ambitious, well-planned and comprehensive profiling of serum proteins that have altered expression in patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).
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