Success of GDNF-based treatment of Hirschsprung disease depends on NCAM1 signaling and various subtypes of enteric neural progenitors
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Hirschsprung disease (HSCR) is a deadly congenital disorder where the enteric nervous system (ENS) is absent from the distal bowel. Current surgical treatment is generally life-saving but is often accompanied by long-term bowel problems and comorbidities. As alternative, we are developing a regenerative therapy based on rectal administration of the neurotrophic factor GDNF. We previously showed that administering GDNF enemas to HSCR mice soon after birth suffice to permanently induce a new ENS from tissue-resident neural progenitors. Here, we clarify the underlying mechanism using single-cell transcriptomics, signal transduction inhibitors and cell lineage tracing tools. Notably, we found that the neurogenic effect of GDNF is mediated by NCAM1, rather than by its canonical signaling receptor RET. We also unveiled the existence of multiple differentiation pathways that involve a larger than expected repertoire of tissue-resident neural progenitors, including a surprising one not derived from the usual neural crest.
