Metaproteomics revealing cyanide bioremediators in effluents from artisanal cassava processing in the Brazilian Amazon

Cassava is widely used and processed for food in several countries. During artisanal processing for flour production, liquid waste containing cyanide may be disposed of inappropriately, contaminating soils, rivers and streams. Various microorganisms must proliferate and carry out fermentation from this waste. The study was carried out on a family farm in the municipality of Bragança, in the state of Pará, Brazil. Samples were selected from the effluents generated in four stages (fermentation tank, post-fermentation washing water tank, washing/pressing water tank ( manipueira ) and effluent lagoon). The aim of this study is therefore to track down microorganisms and their enzymes, through metaproteomics, at all stages of cassava processing up to the elimination of wastewater discharges, using the metaproteomics technique in order to track down potential cyanide bioremediators adapted to local environmental conditions. The samples were extracted using the Phenol-SDS protocol, washed, digested and submitted to a high-resolution mass spectrometer coupled to liquid chromatography and subsequently analyzed using sensitive software. Microorganisms with potential cyanide bioremediation potential were identified ( Bacillus subitilis, Pseudomonas putida and Levilactobacillus brevis ), as well as nitrogen fixers involved in bioremediation. The identified peptides analyzed showed proteins involved in: DNA repair; glycosylation; phosphorylation; CAZY proteins, among others. These findings provide a foundation for clean biotechnological applications aimed at treating cyanide-contaminated effluents in artisanal cassava processing in the Amazonian territory.