The Dynamic Nature of Genetic Risk for Schizophrenia Within Genes Regulated by FOXP1 During Neurodevelopment
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FOXP1 (Forkhead-box protein P1) is a crucial transcription factor in neural development and is associated with schizophrenia (SCZ). FOXP1 -regulated genes may contribute to genetic risk of SCZ and this may vary across different stages of neurodevelopment. We analyzed transcriptomic data from mouse and human models of FOXP1 loss-of-function across prenatal and postnatal developmental stages, including neural stem cells from embryonic mice (E14.5) and human brain organoids (equivalent to second trimester), and cortical tissues from different mouse postnatal stages P0, P7, and P47. P0 in mice corresponds to the third trimester in humans, while P7 and P47 represent early childhood and adolescence, respectively. The effect of FOXP1 disruption on gene expression in cortical tissues/cells was assayed using RNA-seq, including time-course and pairwise gene expression analysis. Linkage disequilibrium score regression assessed if FOXP1 -regulated genes were enriched for SCZ heritability. Gene-set enrichment analysis investigated if FOXP1 -regulated genes were enriched for SCZ-associated genes reported as differentially expressed in single cortical cell studies. SynGO analysis mapped FOXP1 -regulated genes to synaptic locations and functions. FOXP1 -regulated genes are enriched for SCZ heritability, with significant results for E14.5, P7 and P47 but not P0. The P7 gene-set showed the strongest enrichment for SCZ-associated genes from single cortical cell studies. FOXP1 -regulated genes at both P7 and P47 were involved in multiple synaptic functions and were mainly enriched within glutamatergic excitatory neurons, with P47 also showing enrichment within GABAergic inhibitory neurons across regions of the postnatal cortex. Prenatal FOXP1 -regulated genes were enriched in progenitor cells and also mapped to the synapse. Genetic risk for SCZ within FOXP1 -regulated genes follows a dynamic trajectory across developmental stages, showing stronger effects at timepoints that map to early childhood, followed by adolescence and second trimester.
Author Summary
Schizophrenia is a complex disorder caused by many genes. While existing treatments can help manage some positive symptoms, such as hallucinations and delusions, they are not effective in treating other life-limiting symptom areas. As a result, individuals with schizophrenia continue to face significant challenges, including disability, unemployment, homelessness, and social isolation. Genome-wide association studies of schizophrenia have been effective at identifying individual SNPs and genes that contribute to these phenotypes but have struggled to immediately uncover the bigger picture of the underlying biology of the disorder. Here we take an individual gene associated with schizophrenia risk, FOXP1 , which is an important regulator of brain development. Using functional genomics data from models where FOXP1 has been disrupted, we identified sets of genes regulated by FOXP1 across different developmental stages, focusing on the cortical region of the brain. Our findings reveal that FOXP1 -regulated genes are most strongly associated with schizophrenia during early childhood and adolescence. These genes are associated with synaptic components, including presynaptic and postsynaptic structures. By integrating developmental timing, cell type specificity, and functional pathways, this study provides valuable insights into the molecular mechanisms underlying schizophrenia.
