Subgingival Microbiome of Type 2 diabetic subjects associated with Periodontal Severity and Metabolic Condition
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Type 2 diabetes and its risk factors such as dyslipidemia and/or obesity enhance severe periodontitis with a poorly understood microbiome. Hence, the purpose of the present study was to describe and compare the subgingival microbiomes of Type 2 diabetes subjects classified by periodontal diseases, to determine the association between periodontal severity and metabolic condition. 71 DNA subgingival samples of 36 subjects were evaluated by Human Oral Microbiome Identification Microarray using 16S rRNA gene-probe. Blood chemistries were obtained to test hemoglobin, and serum lipid profile. Obesity classification was obtained by Body-Mass-Index. Kruskal-Wallis, Mann-Whitney, Spearman’s correlation, and Multivariate ordinal logistic-regression (periodontal-severity) test were applied (IBM-SPSS-21/Stata 17). Our results suggest samples of subjects with Gingivitis ( n= 12) presented with higher Bacteroidetes proportion vs. Generalized Periodontitis grouped by severity Stage-I ( n =17, NS), Stage-II ( n =30, p< 0.05), and Stage-III ( n =12, p< 0.05); and Generalized Periodontitis Stage-II presented higher Firmicutes proportion ( p< 0.05_MW_ vs._ Gingivitis). Human-Microbial-Taxon presented significant higher Score-levels (Levels), frequency (%), or Odds Ratio (OR) of * 1 Cardiobacterium valvarum _HMT-540, Fusobacterium Cluster-AE01 (Generalized Periodontitis-Stage-I vs. Gingivitis_ p< 0.001_Levels, OR: 9.1 and 4.7), * 2 Porphyromonas gingivalis HMT-619 (OR: 4.7), Ɨ Porphyromonas pasteri _HMT-279 (Gingivitis vs. Generalized Periodontitis-Stage-I and II_ p< 0.01/ p< 0.001_Levels), * 3 Streptococcus constellatus _HMT-576/ Streptococcus intermedius _HMT-644 (Generalized Periodontitis-Stages-II, III vs. Gingivitis_ p< 0.05_%), and † Saccharibacteria (TM7)[G-1]_ bacterium_ HMT-347,350_Gingivitis_ p< 0.05_Levels), some of the Human-Microbial-Taxon identified, presented correlation with total triglycerides (≥150mg/dl, Ɨ 0.521), total lipids (≥800mg/dl, † 0.478), and obesity (* 1 0.279, * 2 0.280, * 3 0.312). The microbiome of Type 2 diabetes subjects with gingivitis presented the classical microbial profile with representative pathogenic species, while the Generalized Periodontitis Stage I-II subjects presented a microbiome with representative putative and saccharolytic species, some of them strongly associated with the poor control of lipid profile or obesity, and to periodontal-severity.
