Rheinheimera palamurensis sp. a novel bacterium isolated from drinking water resources

A bacterial strain designated NG3 ^T was isolated from a drinking water samples collected from Nagar Kurnool, Telangana State, India (16° 29’ 14.5356’’ N ; 78° 18’ 33.966’’) and characterized using a polyphasic taxonomic approach, Strain NG3 ^T was Gram-negative, aerobic, non pigmented and motile by means of a single polar flagellum. Growth occurred at 18 - 37° C (optimum 27 - 32° C), pH at 6.5 – 8.0 (Optimum 7.0 – 7.5) and in the presence of 0-2% NaCl (Optimum 0.7-1.0%), Phylogenetic analyses based on 16S rRNA gene sequence showed that strain NG3 ^T belonged to the genus Rheincheimera and its most closely related neighbor was Rheinheimera aquatica GR5 ^T (GQ168584) with sequence similarity of 99 %. The cellular fatty acid composition of strain NG3 ^T showed a spectrum of 11 fatty acids with a pronounced dominance of saturated fatty acids (82.7 %), including a high abundance of C16 : 0, C18 : 1ω7c, and C12 : 0 3-OH. The major respiratory quinone was MK-7 and Q-8. The DNA G+C content of the genomic DNA was 48.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, aminolipid and two uncharacterized phospholipids. The DNA–DNA relatedness of strain NG3 ^T with respect to recognized members of the genus Rheinheimera was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NG3 ^T represents a novel species in the genus Rheinheimera , for which the name Rheinheimera palamurensis sp. nov. was proposed. The type strain is NG3 ^T (=GU566360=JCM16716=KCTC23111).