Evolution of sensory systems underlies the emergence of predatory feeding behaviours in nematodes

Sensory systems are the primary interface between an organism and its environment with changes in selectivity or sensitivity representing key events in behavioural evolution. Here, we explored the molecular modifications influencing sensory perception across the nematode phyla. Pristionchus pacificus is a predatory species and has evolved contact-dependent sensing and teeth-like structures to attack prey. Using mutants defective for mechanosensory neuron function, we found an expanded role for this sensory modality in efficient predation alongside its canonical function in sensing aversive touch. To identify the precise mechanism involved in this tactile divergence we generated mutations in 26 canonical mechanosensory genes and tested their function using a combination of behavioural assays, automated behavioural tracking and machine learning. While mechanosensory defects were observed in several mutants, Ppa-mec-6 mutants specifically also induced predation deficiencies. Previously, a similar phenotype was observed in a chemosensory defective mutant and we found a synergistic influence on predation in mutants lacking both sensory inputs. Importantly, both chemosensory and mechanosensory receptor expression converge on the same environmentally exposed IL2 neurons revealing these as the primary mechanism for sensing prey. Thus, predation evolved through the co-option of both mechanosensory and chemosensory systems which act synergistically to shape the evolution of complex behavioural traits.