Type I Interferon production in myeloid cells is regulated by factors independent of Ptpn22

The immune regulatory gene PTPN22 is expressed in all immune cells and encodes Lyp in humans and the ortholog PEP in mice. The PTPN22 alternative allele, 1858C>T, is expressed in 5-15% of the North American population and is strongly associated with the development of autoimmune disease while simultaneously capable of providing protection during virus infection and cancer. In murine models, significant progress has been made in elucidating the molecular mechanisms that PEP and its pro-autoimmune variant (PEP-R619W) modulate T cell function, yet their influence on non-T cell pathways, such as antigen presenting cell cytokine production, remains less defined. Previously, it was reported that PEP promotes type I interferon (IFN-I) production in dendritic cells (DCs) and macrophages following TLR4 stimulus. Here, we show that contrary to previous results, both PEP-WT and the PEP-R619W variant do not promote IFN-I production in DCs and macrophages following exposure to LPS, 3p-hpRNA, or coronavirus MHV A59. We attribute the prior findings to mouse strain-specific differences and conclude that something other than PEP may be regulating IFN-I production in these studies. We further show that PEP and its R619W variant distinctly modulate the production of TNFα, IL-12 and IL-2, in DCs following LPS stimulus. Taken together, our results challenge the current understanding of PEP’s role during inflammation while providing new insight into how the PEP-R619W variant may alter myeloid cell function during disease.