Isolation and Characterization of Extracellular Vesicles and Non-Vesicular Extracellular Particles from Mouse Tissues
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Extracellular vesicles (EVs) and non-vesicular extracellular particles (NVEPs) have been recently discovered as part of the diverse components of the secretome. EVs and NVEPs have been shown to differ not only in size and morphology but also in their cargo and biological functions. NVEPs are a newly discovered group of multimolecular assemblies with potential roles in physiological and pathological states. However, very little is known about this new class of particles. Here, for the first time, we present a method for simultaneously isolating and characterizing EVs and NVEPs from primary mouse tissues.
