sedimix : A workflow for the analysis of hominin nuclear DNA sequences from sediments
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Summary
Sediment DNA––the ability to extract DNA from archaeological sediments–– is an exciting new frontier in ancient DNA research, offering the potential to study individuals at a given archaeological site without destructive sampling. In recent years, several studies have demonstrated the promise of this approach by recovering hominin DNA from prehistoric sediments, including those dating back to the Middle or Late Pleistocene. However, a lack of open-source workflows for analysis of hominin sediment DNA samples poses a challenge for data processing and reproducibility of findings across studies. Here we introduce a snakemake workflow, sedimix , for processing genomic sequences from archaeological sediment DNA samples to identify hominin sequences and generate relevant summary statistics to assess the reliability of the pipeline. By performing simulations and comparing to published studies, we show that sedimix has high sensitivity and precision. sedimix offers a reliable and adaptable framework to aid in the analysis of sediment DNA datasets and improve reproducibility across studies.
Availability and Implementation
sedimix is available as an open-source software with the associated code and user manual available at https://github.com/jierui-cell/sedimix
Contact
Jierui Xu ( jierui.xu@berkeley.edu )
Supplementary information
Supplementary data are available at Bioinformatics online
