Precision in Spinal Cord Injury Research: A Novel Electromagnetic Impactor for a Consistent Porcine Model
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Purpose
Replicating spinal cord injury (SCI) in large animals is necessary for evaluating therapeutics for potential human translation, yet there is currently no commercial, standardized device for inducing SCI. We present the fabrication and testing of a custom impactor device for producing repeatable contusion SCI in porcine models.
Methods
We first designed and built the device. Mechanical modeling was subsequently utilized to calibrate our benchtop testing setup. Benchtop verification was performed to measure impact force post calibration. We then used the device to generate a contusion SCI model in 2 pigs and the results were compared to an uninjured pig. Intraoperative ultrasound was used to visualize a hematoma in the injured spinal cord. Hematoxylin-eosin (H&E) and Masson’s trichrome staining were used to confirm injury presence on ex vivo spinal cord samples.
Results
Mechanical modeling forces matched benchtop impact forces within 1.4 N, indicating successful calibration of the testing setup. Our device demonstrated repeatability and the potential for modulating injury severity on the benchtop. Impactor forces were demonstrated across a range from 12.8 to 67.6 N, with variability remaining within 0.2 to 0.7 N standard deviation. The device induced two contusion injuries of different severity in vivo , confirmed by intraoperative ultrasound imaging and post-excision histology of the spinal cord.
Conclusion
Our impactor device is a major advancement towards producing repeatable and titratable contusions in large animal SCI models.
