A Multiplex qPCR to Differentiate Monkeypox Virus Clades

  1. Christopher T. Williams
  2. Alessandra Romero-Ramirez
  3. Adeleye Adesola Semiu
  4. Samuel Oluwafunmbi Ifabumuyi
  5. Susan Gould
  6. Dominic Wooding
  7. Collette Allen
  8. Anushri Somasundaran
  9. Nicodemus Nnabuike Mkpuma
  10. Dorcas Gado
  11. Jolly Amoche Adole
  12. Abdulakeem Eniola Amoo
  13. Abisola Ajoke Adeyemi
  14. Laure Bosquillon de Jarcy
  15. Christine Goffinet
  16. Jake Dunning
  17. Malcolm G Semple
  18. ISARIC CCP UK investigators
  19. Esto Bahizire
  20. Afolabi Akinpelu
  21. Thomas E Fletcher
  22. Ana Cubas-Atienzar
  23. Cristina Leggio
  24. Adeyinka Adedeji
  25. Adesuyi A. Omoare
  26. Thomas Edwards
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Abstract

We designed a multiplex qPCR to differentiate monkeypox virus clades. In evaluations using clinical samples collected in the UK and Nigeria, the assay had an overall sensitivity of 78% (95% CI: 67.67% to 86.14%) and specificity of 94% (95% CI: 80.84% to 99.30%); for samples under Ct35 sensitivity was 98% (95% CI: 91.72% to 99.96%) and specificity was 94% (95% CI: 80.84% to 99.30%).

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  1. Version published to 10.1101/2025.02.07.25321854v1 on medRxiv