Complete mitochondrial genome assembly of two searobin species (genus Prionotus ) and their phylogenetic relationships (Triglidae: Prionotinae)
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Background
The genus Prionotus “searobins” are marine ray-finned fish of the subfamily Prionotinae that have evolved distinctive morphological features and specialized behavior for walking over the seafloor detecting buried preys, making them excellent candidates for biological models in evolutionary genetics studies. Despite that, their phylogenetic relationships are still not well understood. Here, we assembled the first complete mitochondrial genomes of two searobin species namely Prionotus alatus and P. stephanophrys and performed a comparative mitogenome examination including the first phylogenetic analysis of Prionotinae based on complete mitogenomic information.
Methods and results
Both mitogenomes were assembled using Illumina genomic reads mined from the GenBank database. Single circular contigs of 16,602 and 16,896 bp were obtained for P. alatus and P. stephanophrys respectively. Both mitogenomes displayed a standard vertebrate organization (13 PCGs, 2 rRNAs, 22 tRNAs, and a putative control region) except for a third tRNA-Leu and an extra non-coding region detected in P. stephanophrys . A Bayesian phylogenetic analysis recovered all Prionotus species into a monophyletic clade within Prionotinae, including the sister taxon Bellator grouped in a discrete subclade. Species of the subfamily Triglinae were resolved in a basal monophyletic group within Triglidae.
Conclusion
The results presented herein represent an important step into a deeper understanding of the phylogenetic relationships and evolution of Prionotus . Taxonomic clarification may also support future management and conservation efforts of these economically valuable species.
