scRepertoire 2: Enhanced and Efficient Toolkit for Single-Cell Immune Profiling

Single-cell adaptive immune receptor repertoire sequencing (scAIRR-seq) and single-cell RNA sequencing (scRNA-seq) provide a transformative approach to profiling immune responses at unprecedented resolution across diverse pathophysiologic contexts. This work presents scRepertoire 2, a substantial update to our R package for analyzing and visualizing single-cell immune receptor data. This new version introduces an array of features designed to enhance both the depth and breadth of immune receptor analysis, including improved workflows for clonotype tracking, repertoire diversity metrics, and novel visualization modules that facilitate longitudinal and comparative studies. Additionally, scRepertoire 2 offers seamless integration with contemporary single-cell analysis frameworks like Seurat and SingleCellExperiment, allowing users to conduct end-to-end immune profiling with transcriptomic data. Performance optimizations in scRepertoire 2 substantially reduce computational time and memory usage, addressing the demands of the ever-increasing size and scale of single-cell studies. This release marks an advancement in single-cell immunogenomics, equipping researchers with a robust toolset to uncover immune dynamics in health and disease.