The inhibitory effects of Remodelin on murine myoblasts differentiation

Myoblasts differentiation is a highly regulated and complex process leading to the formation of fused and aligned mature myotubes. Growing interest in the role of epigenetics in muscle differentiation has highlighted epi-modulators as crucial regulators of this process. Our in vitro study aimed to explore the potential effects of Remodelin, a selective inhibitor of the acetyltransferase Nat10, on myoblasts differentiation.

We cultivated and differentiated murine C2C12 myoblasts on ultra-compliant gelatin for up to 16 days, and treated them with Remodelin. We used confocal microscopy, together with RNAseq and proteomics analyses, to examine the impact of Remodelin on myotube formation.

Remodelin impaired myotube organization and maturation, and proper morphology compared to untreated cells. Additionally, no significant twitching was observed upon Remodelin treatment. Intersection of transcriptomics and proteomics analyses confirmed that Remodelin effectively slowed myotube formation. Overall, these results suggest that Remodelin broadly affects the regulatory networks involved in skeletal muscle differentiation.