High-throughput two-photon volumetric brain imaging in freely moving mice
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Imaging neural activities across large volumes at subcellular resolution without impeding animal behaviors remains difficult. Here we introduce a high-throughput miniature Bessel-beam two-photon microscope capable of imaging calcium dynamics from neurons and dendrites over a volume of 420 × 420 × 80 μm³ during free behavior. We share full description of the microscope and demonstrate calcium imaging of more than 1000 neurons at a time in freely moving mice.
