Persistent Na ⁺ current couples spreading depolarization to seizures in Scn8a gain of function mice

Spreading depolarization (SD) is a slowly propagating wave of massive cellular depolarization that transiently impairs the function of affected brain regions. While SD typically arises as an isolated hemispheric event, we previously reported that reducing M-type potassium current (I _KM ) by ablation of Kcnq2 in forebrain excitatory neurons results in tightly coupled spontaneous bilateral seizure-SD complexes in the awake mouse cortex. Here we find that enhanced persistent Na ⁺ current due to gain-of-function (GOF) mutations in Scn8a (N1768D/+, hereafter D/+) produces a similar compound cortical excitability phenotype. Chronic DC-band EEG recording detected spontaneous bilateral seizure-SD complexes accompanied by seizures with a profound tonic component, which occurs predominantly during the light phase and were detected in the mutant mice across ages between P40-100. Laser speckle contrast imaging of cerebral blood flow dynamics resolved SD as bilateral wave of hypoperfusion and subsequent hour-lasting hypoperfusion in Scn8a ^D/+ cortex in awake head-restrained mice subjected to a subconvulsive PTZ. Subcortical recordings in freely moving mice revealed that approximately half of the spontaneous cortical seizure-SD complexes arose with concurrent SD-like depolarization in the thalamus and delayed depolarization in the striatum. In contrast, SD-like DC potential shifts were rarely detected in the hippocampus or upper pons. Consistent with the high spontaneous incidence in vivo , cortical slices from Scn8a ^D/+ mice showed a raised SD susceptibility, and pharmacological inhibition of persistent Na ⁺ current (I _NaP ), which is enhanced in Scn8a ^D/+ neurons, inhibited SD generation in cortical slices ex vivo , indicating that I _NaP contributes to SD susceptibility. Ex vivo Ca ²⁺ imaging studies using acute brain slices expressing genetic Ca ²⁺ sensor (Thy1-GCAMP6s) demonstrated that pharmacological activation of I _KM suppressed Ca ²⁺ spikes and SD, whereas I _KM inhibitor drastically increased the frequency of Ca ²⁺ spikes in the hippocampus of Scn8a ^D/+ mice, but not in WT, suggesting that I _KM restrains the hyperexcitability resulting from Scn8a GOF mutation. Together, our study identifies a cortical SD phenotype in Scn8a GOF mice shared with the Kcnq2 - cKO model of developmental epileptic encephalopathy and reveals that an imbalance of non-inactivating inward and outward membrane currents bidirectionally modulates spatiotemporal SD susceptibility.