Immunopeptidomics informs discovery and delivery of Mycobacterium tuberculosis MHC-II antigens for vaccine design
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (Rapid Reviews Infectious Diseases)
Abstract
No currently licensed vaccine reliably prevents pulmonary tuberculosis (TB), a leading cause of infectious disease mortality. Developing effective new vaccines will require identifying which of the roughly 4000 proteins in the Mycobacterium tuberculosis ( Mtb ) proteome are presented on MHC class II (MHC-II) by infected human phagocytes and can be recognized by CD4+ T cells to mediate protective immunity. Vaccines must also elicit T cell responses recognizing the same peptide-MHC complexes presented by infected cells, and successful presentation of target human MHC-II peptides is currently challenging to evaluate and optimize. Here, we define antigenic targets for TB vaccine development by using mass spectrometry (MS) for proteome-wide discovery of Mtb epitopes presented on MHC-II by infected human cells. We next iteratively design and evaluate candidate mRNA vaccine immunogens, revealing design principles that enhance presentation of target MHC-II peptides. Our results will inform the development of new TB vaccine candidates.
Article activity feed
-
Hazem Abdelaal
Review 2: "Immunopeptidomics Informs Discovery and Delivery of Mycobacterium Tuberculosis MHC-II Antigens for Vaccine Design"
Reviewers found the preprint potentially informative. They highlighted the extensive characterization of the M. tuberculosis proteome and potential new vaccine targets the study suggested.
-
William Davis
Review 1: "Immunopeptidomics Informs Discovery and Delivery of Mycobacterium Tuberculosis MHC-II Antigens for Vaccine Design"
Reviewers found the preprint potentially informative. They highlighted the extensive characterization of the M. tuberculosis proteome and potential new vaccine targets the study suggested.
-
Strength of evidence
Reviewers: W Davis (Washington State University) | 📒📒📒 ◻️◻️
H Abdelaal (Seattle Children's Research Institute) | 📒📒📒◻️◻️ -
