A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo

  1. Takaki Yahiro
  2. Landon Bayless-Edwards
  3. James A. Jones
  4. Lei Ma
  5. Maozhen Qin
  6. Tianyi Mao
  7. Haining Zhong
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Abstract

We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Förster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables in vivo imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.

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  1. Version published to 10.1101/2024.07.19.604387v1 on bioRxiv