C-reactive protein-based tuberculosis triage testing: a multi-country diagnostic accuracy study
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (Rapid Reviews Infectious Diseases)
Abstract
Rationale
C-reactive protein (CRP)-based tuberculosis (TB) screening is recommended for people with HIV (PWH). However, its performance among people without HIV and in diverse settings is unknown.
Objectives
In a multi-country study, we aimed to determine whether CRP meets the minimum accuracy targets (sensitivity ≥90%, specificity ≥70%) for an effective TB triage test.
Methods/Measurements
Consecutive outpatient adults with cough ≥2 weeks from five TB endemic countries in Africa and Asia had baseline blood collected for point-of-care CRP testing and HIV and diabetes screening. Sputum samples were collected for Xpert MTB/RIF Ultra (Xpert) testing and culture. CRP sensitivity and specificity (5 mg/L cut-point) was determined in reference to sputum test results and compared by country, sex, and HIV and diabetes status. Variables affecting CRP performance were identified using a multivariate receiver operating characteristic (ROC) regression model.
Results
Among 2904 participants, of whom 613 (21%) had microbiologically-confirmed TB, CRP sensitivity was 84% (95% CI: 81-87%) and specificity was 61% (95% CI: 59-63%). CRP accuracy varied geographically, with higher sensitivity in African countries (≥91%) than Asian countries (64-82%). Sensitivity was higher among men than women (87% vs. 79%, difference +8%, 95% CI: 1-15%) and specificity was higher among people without HIV than PWH (64% vs. 45%, difference +19%, 95% CI: 13-25%). ROC regression identified country and measures of TB disease severity as predictors of CRP performance.
Conclusions
Overall, CRP did not achieve the minimum accuracy targets and its performance varied by setting and in some sub-groups, likely reflecting population differences in mycobacterial load.
Article activity feed
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Delia Goletti
Review 2: "C-reactive Protein-Based Tuberculosis Triage Testing: A Multi-Country Diagnostic Accuracy Study"
Reviewers found this study robust and informative, suggesting only minor improvements, such as following up with individuals with a negative sputum analysis.
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Norbert Heinrich
Review 1: "C-reactive Protein-Based Tuberculosis Triage Testing: A Multi-Country Diagnostic Accuracy Study"
Reviewers found this study robust and informative, suggesting only minor improvements, such as following up with individuals with a negative sputum analysis.
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Strength of evidence
Reviewers: N Heinrich (Ludwig-Maximilians-University of Munich) | 📘📘📘📘📘
D Goletti (National Institute for Infectious Deseases) | 📘📘📘📘📘 -
