A Step-Emulsion Based Digital-RPA for Pathogenic Bacteria Detection
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Foodborne bacteria are major causes that affect human health. Development of new method that could achieve Rapid, sensitive and quantitative detection of pathogen is urgently needed. In this research, a step emulsion microfluidic,combined with droplet-based digital-RPA, was developed to detect Vibrio parahaemolyticus , a major seafood-borne pathogenic bacteria. Specific and rapid detection of Vibrio parahaemolyticus in 30 min has been achieved by this new device, with a detection limit of 10 CFU/μL, about 10-times lower than classical tube-based RPA. This device was demonstrated to be a promising tool for detection of pathogenic bacteria.