Single-Molecule FRET and Tracking of Transfected Biomolecules in Living Cells
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Proteins and DNA in cells exhibit different conformational states, which are influenced by dynamic interactions with other biomolecules. All these interactions are affected by the molecules’ localization within the cell, i.e., their compartmentalization. Such, in cellula, compartment-specific dynamics is difficult to measure, because of limitations in instrumentation, autofluorescence of cells, and the necessity to track diffusing molecules. Here, we present a bottom-up engineering approach, which allows us to track transfected proteins in cellula and to analyze time-resolved single-molecule FRET efficiencies. This has been achieved by alternating laser excitation (ALEX) based three-channel (donor, acceptor and FRET) tracking with a live-cell HILO microscope. We validate our strategy by characterizing long-term static-FRET traces of customized DNA with known dye positions. We utilize two different transfection strategies, namely a biological (Streptolysin-O toxin protein) and a physical one (Microinjection). By comparing in vitro and in cellula measurements we show that the cellular environment in this case changes the FRET efficiency by about 25%. In addition, we evaluate single-molecule FRET traces for the heat shock protein Hsp90 in cellula. The obtained FRET efficiency distribution is largely consistent with known Hsp90 structures and in vitro distributions, but also shows some clear differences. Altogether, we show that FRET-TTB (Förster Resonance Energy Transfer-Tracking of Transfected Biomolecules) opens the path to study protein state changes of transfected biomolecules in cellula, including time-resolved cellular localization.
Significance
Inside cells, proteins and DNA can change shape depending on their environment and their interactions. Studying these changes for single biomolecules is challenging because they move around in the cell and the cell environment makes it hard to see them clearly. Here, we demonstrate a new technique called FRET-TTB that enables us to track individual proteins inside living cells over time using single-molecule fluorescence microscopy. We validate the approach with custom DNA and apply it to study the heat shock protein Hsp90. Our results show that this method can reveal changes in protein conformation and location within living cells.
