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  3. SciScore for 10.1101/2022.05.26.493517: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Antibodies
    SentencesResources
    Spike proteins were captured through their C-terminal His-tag over an anti-His antibody surface.
    anti-His
    suggested: None
    For each residue within the RBD, the frequency of antibody recognition was calculated as the number of contact antibodies32.
    antibodies32
    suggested: None
    The structures of antibody-spike complexes for modeling were also obtained from PDB (7L5B (2-15), 6XDG (REGN10933), and 7KMG (LY-CoV555)).
    REGN10933
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Heavy chain variable (VH) and light chain variable (VL) genes for each antibody were synthesized (GenScript), then transfected into Expi293 cells (Thermo Fisher Scientific), and purified from the supernatant by affinity purification using rProtein A Sepharose (GE).
    Expi293
    suggested: RRID:CVCL_D615)
    A14527); Vero-E6 cells were obtained from the ATCC (
    Vero-E6
    suggested: None
    CRL-1586); HEK293T cells were obtained from the ATCC (CRL-3216).
    HEK293T
    suggested: None
    Software and Algorithms
    SentencesResources
    Neutralization curves and IC50 values were derived by fitting a nonlinear five-parameter doseโ€“response curve to the data in GraphPad Prism v.9.2.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    PyMOL v.
    PyMOL
    suggested: (PyMOL, RRID:SCR_000305)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).


    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.


    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.


    Results from JetFighter: We did not find any issues relating to colormaps.


    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.


    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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