Reduced Neutralization of SARS-CoV-2 Omicron Variant in Sera from SARS-CoV-1 Survivors after 3-dose of Vaccination

  1. Xuesen Zhao
  2. Danying Chen
  3. Xiaohua Hao
  4. Yaruo Qiu
  5. Juan Du
  6. Yuanyuan Zhang
  7. Fan Xiao
  8. Xinglin Li
  9. Yanjun Song
  10. Rui Song
  11. Xi Wang
  12. Ronghua Jin

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Abstract

Recent studies found that Omicron variant escapes vaccine-elicited immunity. Interestingly, potent cross-clade pan-sarbecovirus neutralizing antibodies were found in survivors of the infection by SARS-CoV-1 after BNT162b2 mRNA vaccination ( N Engl J Med. 2021 Oct 7;385(15):1401-1406 ). These pan-sarbecovirus neutralizing antibodies were observed to efficiently neutralize the infection driven by the S protein from both SARS-CoV and multiple SARS-CoV-2 variants of concern (VOC) including B.1.1.7 (Alpha), B.1.351 (Beta), and B.1.617.2 (Delta). However, whether these cross-reactive antibodies could neutralize the Omicron variant is still unknown. Based on the data collected from a cohort of SARS-CoV-1 survivors received 3-dose of immunization, our studies reported herein showed that a high level of neutralizing antibodies against both SARS-CoV-1 and SARS-CoV-2 were elicited by a 3rd-dose of booster vaccination of protein subunit vaccine ZF2001. However, a dramatically reduced neutralization of SARS-CoV-2 Omicron Variant (B.1.1.529) is observed in sera from these SARS-CoV-1 survivors received 3-dose of Vaccination. Our results indicates that the rapid development of pan-variant adapted vaccines is warranted.

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  1. ScreenIT

    SciScore for 10.1101/2022.05.21.492903: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Ethical statement: The study protocol was approved by the Ethics Committee of the Institute of Beijing Ditan Hospital, Capital Medical University (IRB#2021-(024)-02).
    Consent: Written informed consent was obtained from all participants before the enrollment.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    HEK-293T cells were transfected with the plasmids expressing different S protein respectively.
    HEK-293T
    suggested: None
    Recombinant DNA
    SentencesResources
    Cell transfection and pseudotyped virus production: The pSectag2 vector was used to construct recombinant plasmid of codon optimized spike proteins of SARS-CoV-2 prototype (Wuhan-1 reference strain containing D614G mutation) and variants, with a 19 amino acid truncation at the C-terminus of the spike protein1 (with mutations shown in Fig.
    pSectag2
    suggested: None
    Software and Algorithms
    SentencesResources
    pVNT50 was interpolated from the neutralization curves determined using the log(inhibitor) vs. normalized response -- Variable slope fit using automatic outlier detection in GraphPad Prism Software.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1101/2022.05.21.492903v1 on bioRxiv