mRNA-1273 but not BNT162b2 induces antibodies against polyethylene glycol (PEG) contained in mRNA-based vaccine formulations
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SciScore for 10.1101/2022.04.15.22273914: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Study participants and human samples: Samples were collected as part of our ongoing institutional review board–approved, longitudinal observational studies (Study ID-20-00442: 64 participants; Study ID-16-01215: one participant).
Consent: All participants signed informed consents prior to data and sample collection.Sex as a biological variable The SARS-CoV-2 mRNA vaccine side effects reported from the participants selected based on their vaccine associated side effects were generally mild to moderate and self-limiting (e.g., injection site pain/swelling, fever, fatigue, etc.) with a subset of participants (N: 8, all females, three received BNT162b2 and five received mRNA-1273) reporting … SciScore for 10.1101/2022.04.15.22273914: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Study participants and human samples: Samples were collected as part of our ongoing institutional review board–approved, longitudinal observational studies (Study ID-20-00442: 64 participants; Study ID-16-01215: one participant).
Consent: All participants signed informed consents prior to data and sample collection.Sex as a biological variable The SARS-CoV-2 mRNA vaccine side effects reported from the participants selected based on their vaccine associated side effects were generally mild to moderate and self-limiting (e.g., injection site pain/swelling, fever, fatigue, etc.) with a subset of participants (N: 8, all females, three received BNT162b2 and five received mRNA-1273) reporting delayed onset, injection site rashes, redness or swelling. Randomization not detected. Blinding Samples were coded and analyzed in a blinded manner. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources After 2 h incubation, plates were washed three times with PBS and then incubated for 1 hour with anti-human IgG (Fab-specific) horse radish peroxidase (HRP) secondary antibody produced in goat (Sigma-Aldrich), or IgM-HRP (Southern Biotech) at a 1:3000 in 1% milk PBS. anti-human IgGsuggested: NoneSpecific spike/PEG-IgE antibodies were assessed by incubating with an anti-IgE HRP conjugated antibody (Invitrogen) for 1 h at a 1:2000 dilution. anti-IgE HRPsuggested: NoneFor the BSA control, plates were incubated with serial dilutions (2-fold) of an anti-albumin (bovine serum) rabbit IgG fraction (anti-BSA, Invitrogen) starting at a 1:1000 dilution for 1 h, followed by three washes with PBS and addition of a donkey anti-rabbit IgG HRP conjugated antibody (CiteAb) at a 1:1000 dilution. anti-albuminsuggested: Noneanti-BSAsuggested: Noneanti-rabbit IgGsuggested: NoneRecombinant DNA Sentences Resources Briefly, the spike (S) gene sequence (GenBank: MN908947) was cloned into a mammalian expression vector pCAGGs, as described (20, 21). pCAGGssuggested: RRID:Addgene_127347)Software and Algorithms Sentences Resources Statistical analyses: Data plotting and statistical analyses were performed using GraphPad Prism 9 (GraphPad Software, San Diego, CA, USA). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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