Vaccine-induced immune thrombotic thrombocytopenia is mediated by a stereotyped clonotypic antibody
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SciScore for 10.1101/2022.03.28.22272975: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All study subjects gave written informed consent prior to inclusion in the study.
IRB: The study was approved by the Clinical Ethics Committee of the Flinders Medical Centre using de-identified diagnostic sera (approval number 39.034)Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Proteomic profiling serum anti-PF4 antibodies: As denoted in the proteomics workflow (Figure 1A) anti-PF4suggested: None, bead-purified anti-PF4 antibody fraction and unbound fractions using ELISAs coated with individual PF4, SARS-CoV-2 spike S1 and S2 proteins (the Native Antigen Company, UK) … SciScore for 10.1101/2022.03.28.22272975: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All study subjects gave written informed consent prior to inclusion in the study.
IRB: The study was approved by the Clinical Ethics Committee of the Flinders Medical Centre using de-identified diagnostic sera (approval number 39.034)Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Proteomic profiling serum anti-PF4 antibodies: As denoted in the proteomics workflow (Figure 1A) anti-PF4suggested: None, bead-purified anti-PF4 antibody fraction and unbound fractions using ELISAs coated with individual PF4, SARS-CoV-2 spike S1 and S2 proteins (the Native Antigen Company, UK) at 4 μg/ml., purified anti-PF4 IgGs were then separated by SDS-PAGE; heavy- and light-chain bands excised for in-gel digestion with trypsin and chymotrypsin, respectively; and analysis of peptides performed in a state-of-the-art Thermo Orbitrap Fusion Lumos Tribrid mass spectrometer with peptide de novo sequencing and IMGT database matching performed by Peaks Studio XPro as detailed previously 15 with modifications. anti-PF4 IgGssuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
Results from scite Reference Check: We found no unreliable references.
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