Low Risk of Severe Acute Respiratory Syndrome Coronavirus 2 Transmission by Fomites: A Clinical Observational Study in Highly Infectious Coronavirus Disease 2019 Patients

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1. Version published to 10.1093/infdis/jiac170

   May 5, 2022
2. 

   ScreenIT

   Mar 26, 2022

   SciScore for 10.1101/2022.03.22.22272773: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Study design: After written informed consent, two combined nasopharyngeal and oropharyngeal swabs were collected from each patient. IRB: Legal and ethical considerations: The study was conducted according to the revised principles of the Declaration of Helsinki and was approved by the ethics committee of the Ruhr-University Bochum (registration number 20-7065) in November 2020.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	Sample size calculation was performed using G*Power Version 3.1.9.6 for windows [12].
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Total RNA was …

   SciScore for 10.1101/2022.03.22.22272773: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Study design: After written informed consent, two combined nasopharyngeal and oropharyngeal swabs were collected from each patient. IRB: Legal and ethical considerations: The study was conducted according to the revised principles of the Declaration of Helsinki and was approved by the ethics committee of the Ruhr-University Bochum (registration number 20-7065) in November 2020.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	Sample size calculation was performed using G*Power Version 3.1.9.6 for windows [12].
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Total RNA was purified from VeroE6 cells using the RNeasy Mini Kit (QIAGEN®, www.qiagen.com).	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   Statistical analysis was performed using GraphPad Prism version 8.0.0 for Windows (GraphPad Software, San Diego, California USA, www.graphpad.com).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   Sample size calculation was performed using G*Power Version 3.1.9.6 for windows [12].	G*Power suggested: (G*Power, RRID:SCR_013726)
   Sequencing libraries were prepared from 4·5μl cDNA using NEBNext® ARTIC SARS-CoV-2 Library Prep Kit for Illumina sequencing platforms (New England BioLabs® Inc., neb.com, catalog #E7650).	New England BioLabs® suggested: (New England Biolabs, RRID:SCR_013517)
   In addition, variants in spike domains were identified and annotated using Geneious prime 2021.2.2 (https://www.geneious.com).	https://www.geneious.com suggested: (Geneious, RRID:SCR_010519)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   Our study encompasses several limitations. Patients were encouraged to forcefully cough twice to contaminate surfaces. However, we cannot exclude that potentially repeated coughing over a prolonged time results in a more effective virus transfer compared to our controlled conditions. Moreover, sneezing can produce significantly more infectious droplets potentially containing infectious particles, therefore, we cannot exclude potential transmissions via other this route. Furthermore, a selection bias cannot be excluded, and the included patients are not demographically representative. Strengths of the present study include the high viral load of the patients included, a standardized protocol for sample acquisition, laboratory procedures and the inclusion of VoC.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2022.03.22.22272773v1 on medRxiv

   Mar 23, 2022