Durable protection against SARS-CoV-2 Omicron induced by an adjuvanted subunit vaccine

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   ScreenIT

   Mar 23, 2022

   SciScore for 10.1101/2022.03.18.484950: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics

   IACUC: Animal subjects and experimentation: Twenty one male rhesus macaques (Macaca mulatta) of Indian origin, aged 5 – 15 years, including 11 animals from the previous study(Arunachalam et al., 2021) were housed and maintained as per National Institutes of Health (NIH) guidelines at the New Iberia Research Center (NIRC) of the University of Louisiana at Lafayette in accordance with the rules and regulations of the Committee on the Care and Use of Laboratory Animal Resources. Field Sample Permit: For the challenge, the animals were transferred to the Regional Biosafety Level 3 facility at the Tulane National Primate Research Center, where the study was reviewed and approved by the Tulane …

   SciScore for 10.1101/2022.03.18.484950: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Animal subjects and experimentation: Twenty one male rhesus macaques (Macaca mulatta) of Indian origin, aged 5 – 15 years, including 11 animals from the previous study(Arunachalam et al., 2021) were housed and maintained as per National Institutes of Health (NIH) guidelines at the New Iberia Research Center (NIRC) of the University of Louisiana at Lafayette in accordance with the rules and regulations of the Committee on the Care and Use of Laboratory Animal Resources. Field Sample Permit: For the challenge, the animals were transferred to the Regional Biosafety Level 3 facility at the Tulane National Primate Research Center, where the study was reviewed and approved by the Tulane University IACUC (protocol 3930)
   Sex as a biological variable	Animal subjects and experimentation: Twenty one male rhesus macaques (Macaca mulatta) of Indian origin, aged 5 – 15 years, including 11 animals from the previous study(Arunachalam et al., 2021) were housed and maintained as per National Institutes of Health (NIH) guidelines at the New Iberia Research Center (NIRC) of the University of Louisiana at Lafayette in accordance with the rules and regulations of the Committee on the Care and Use of Laboratory Animal Resources.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Cells were incubated with an anti-SARS-CoV spike primary antibody directly conjugated to Alexaflour-647 (CR3022-AF647) for 4 hours at room temperature or overnight at 4° C.	anti-SARS-CoV spike suggested: None
   Antibody half-life calculations: Mixed-effects models implemented in MonolixSuite 2021R1 (Lixoft) were used to estimate the corresponding half-lives of antigen-specific antibodies.	antigen-specific suggested: None
   741172), CD3 BV650 (BD Biosciences, 563916), CD21 PE-CF594 (BD Biosciences, 563474) and Alexa Fluor 488-labeled Beta spike (antibodies-online, ABIN6963740),	CD3 suggested: (BD Biosciences Cat# 563916, RRID:AB_2738486) antibodies-online , ABIN6963740 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   To perform neutralization assay, Vero E6-TMPRSS2-T2A-ACE2 cells (BEI Resources, NIAID; NR-54970) were seeded at a density of 1×104 per well in half area 96-well black opaque plates (Greiner Bio-One) and were grown overnight at 37°C in a 5% CO2 atmosphere.	Vero E6-TMPRSS2-T2A-ACE2 suggested: None
   Viruses and cells: VeroE6-TMPRSS2 cells were described previously and cultured in complete DMEM in the presence of Gibco Puromycin 10 mg/ml (#A11138-03). nCoV/USA_WA1/2020 (WA/1) was propagated from an infectious SARS-CoV-2 clone as previously described(Xie et al., 2020).	VeroE6-TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   Animal subjects and experimentation: Twenty one male rhesus macaques (Macaca mulatta) of Indian origin, aged 5 – 15 years, including 11 animals from the previous study(Arunachalam et al., 2021) were housed and maintained as per National Institutes of Health (NIH) guidelines at the New Iberia Research Center (NIRC) of the University of Louisiana at Lafayette in accordance with the rules and regulations of the Committee on the Care and Use of Laboratory Animal Resources.	NIRC suggested: None
   The FRNT-50 titers were interpolated using a 4-parameter nonlinear regression in GraphPad Prism 9.2.0.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   All flow cytometry data were analysed using Flowjo software v10 (TreeStar Inc.).	Flowjo suggested: (FlowJo, RRID:SCR_008520)
   All the figures were made in GraphPad Prism or R and organized in Adobe Illustrator.	Adobe Illustrator suggested: (Adobe Illustrator, RRID:SCR_010279)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT05007951	Recruiting	Immunogenicity and Safety Study of SK SARS-CoV-2 Recombinant…

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Version published to 10.1101/2022.03.18.484950v1 on bioRxiv

   Mar 20, 2022