Evaluating the transmission risk of SARS-CoV-2 from sewage pollution
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2022.03.16.22272465: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: As this method has only been validated for non-enveloped viruses (34), via RNA detection, to validate it for assessing the presence and infectivity of SARS-CoV-2, 10 l of Milli-Q water was spiked with murine hepatitis virus (MHV-A59) to a final concentration of 1.5 × 104 gc/L, and run through the TFUF to test processing efficiency. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources NCTC 1469 cells were used for propagation and infectivity assays of MHV using the manufacturers protocol using DMEM high glucose … SciScore for 10.1101/2022.03.16.22272465: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: As this method has only been validated for non-enveloped viruses (34), via RNA detection, to validate it for assessing the presence and infectivity of SARS-CoV-2, 10 l of Milli-Q water was spiked with murine hepatitis virus (MHV-A59) to a final concentration of 1.5 × 104 gc/L, and run through the TFUF to test processing efficiency. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources NCTC 1469 cells were used for propagation and infectivity assays of MHV using the manufacturers protocol using DMEM high glucose with 10% horse serum. NCTC 1469suggested: NoneAfter incubation, samples were vortexed, filtered through a 0.22 μM filter and serially diluted to achieve a starting dilution of 200 TCID50/well on Vero E6 cells (41). Vero E6suggested: RRID:CVCL_XD71)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:The positive sample was collected downstream from the largest CSO on the Danube River (Pančevo Bridge), where there is a high dilution potential due to an average discharge of 5,600 m3/s in this section of river (13), highlighting the limitations of point samples from surface water for environmental monitoring. Research suggests that high percentages of enveloped viruses (26%) can adsorb to the solid fraction of wastewater (43), that suspended solids may protect viruses from inactivation (44), and that sediments can provide a source of pathogens to the water column (45). Studying the presence of viruses in sediments, rather than water, may provide greater insight into sites that are susceptible to accumulating and harbouring potential pathogens, rather than taking water column point samples, that can suffer from high variability (46). While most studies have focused on the presence of SARS-CoV-2 RNA in wastewater (reviewed by (5), and river water (13,14), very little is known about the potential survival of SARS-CoV-2 in aquatic environments (47,48). Here we tested whether a validated TFUF protocol for the concentration of human non-enveloped enteric viruses from river water (34) could also be used to concentrate an enveloped betacoronavirus, Murine hepatitis virus (MHV). Considering the rigorous biosafety requirements necessary for working with infectious SARS-CoV-2, surrogate viruses, such as MHV, are useful for assessing method performance during monitoring campaigns (49)....
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
