Aerosolized Ad5-nCoV booster vaccination elicited potent immune response against the SARS-CoV-2 Omicron variant after inactivated COVID-19 vaccine priming

Article activity feed

1. 

   ScreenIT

   Mar 12, 2022

   SciScore for 10.1101/2022.03.08.22271816: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: The protocol was approved by the Ethics Committee of 305 Hospital of PLA.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   RBD-binding IgG assay: RBD-binding IgG antibodies in the heat-inactivated human serum samples and the culture supernatant of PBMCs stimulated for 4 days with R848 + IL-2 were detected with an RBD-binding IgG ELISA kit (Beijing, Kewei).	RBD-binding IgG suggested: None
   The total anti-RBD IgG antibody levels were quantitated in ELISA units (EU) ml-1 by comparison to a reference standard curve created from monoclonal …

   SciScore for 10.1101/2022.03.08.22271816: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: The protocol was approved by the Ethics Committee of 305 Hospital of PLA.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   RBD-binding IgG assay: RBD-binding IgG antibodies in the heat-inactivated human serum samples and the culture supernatant of PBMCs stimulated for 4 days with R848 + IL-2 were detected with an RBD-binding IgG ELISA kit (Beijing, Kewei).	RBD-binding IgG suggested: None
   The total anti-RBD IgG antibody levels were quantitated in ELISA units (EU) ml-1 by comparison to a reference standard curve created from monoclonal antibodies against SARS-CoV-2 RBD.	anti-RBD IgG suggested: None
   PVDF ELISpot plates were coated with a purified anti-human IgG monoclonal antibody (MT91/145), incubated at 4-8 °C overnight, and blocked with RPMI 1640 medium containing 10% fetal bovine serum and 1× penicillin–streptomycin solution (Gibco) for at least 30 min at room temperature.	anti-human IgG suggested: (GenWay Biotech Inc. Cat# 20-272-192774-0.5 ml, RRID:AB_1984561)
   IFNγ spots were detected after the addition of a biotinylated detection antibody (7-B6-1-biotin, 1 μg ml-1) followed by streptavidin-HRP and TMB substrate.	7-B6-1-biotin suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   HEK293T cells were cotransfected with the plasmids pNL4.3-Luc-R-E- and pCAGGS-SWT or pCAGGS-SOmicron with TurboFect transfection reagent (Thermo Scientific).	HEK293T suggested: None
   Each sample was serially diluted 3-fold in duplicate from 1:30 to 1:7290 or 1:21870 in complete DMEM before incubation with the titrated pseudovirus SARS-CoV-2 for 1 hour prior to the addition of 2×104 293T-ACE2 cells.	293T-ACE2 suggested: None
   Recombinant DNA
   Sentences	Resources
   HEK293T cells were cotransfected with the plasmids pNL4.3-Luc-R-E- and pCAGGS-SWT or pCAGGS-SOmicron with TurboFect transfection reagent (Thermo Scientific).	pNL4.3-Luc-R-E- suggested: None pCAGGS-SWT suggested: None pCAGGS-SOmicron suggested: None
   Software and Algorithms
   Sentences	Resources
   The results were analyzed by GraphPad Prism 8.0.2 using 4-PL curve fitting.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   There are some limitations to this study. First, we have not yet developed appropriate assays for mucosal immune responses to deepen our understanding of the immune advantage of aerosolized Ad5-nCoV. Saliva IgA antibodies were detected in some BNT162b2 vaccine recipients(25, 26), but whether IgA was exuded from serum or produced by a local mucosal immune response could not be determined. More assays need to be developed to analyze local mucosal immune responses, including secretory IgA antibodies and local cellular immune responses. Second, we assessed immunogenicity 28 days after booster vaccination, but further development of the immune response and the longevity of the responses remain to be evaluated. The half-life of serum neutralizing antibodies was 69-173 days for two-dose mRNA vaccine recipients and 103 days for SARS-CoV-2 convalescents(27-29). The long-term antibody dynamics of neutralizing antibodies after the booster vaccination should be further investigated. In summary, in the face of waning immunity and the circulation of SARS-CoV-2 variants, neutralizing antibody and T-cell responses were boosted most efficiently with aerosolized Ad5-noV in those who received inactivated vaccines as initial doses. Boosters probably increase vaccine effectiveness against infection with and transmission of the SARS-CoV-2 Omicron variant.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
2. 

   Version published to 10.1101/2022.03.08.22271816 on medRxiv

   Mar 10, 2022