Immunogenicity of an Ad26-based SARS-CoV-2 Omicron Vaccine in Naïve Mice and SARS-CoV-2 Spike Pre-immune Hamsters

Article activity feed

1. 

   ScreenIT

   Mar 7, 2022

   SciScore for 10.1101/2022.03.04.482636: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Euthanasia Agents: Blood samples were collected via the retro-orbital route under isoflurane anesthesia.
   Sex as a biological variable	Female BALB/c mice aged 8-10 weeks at the start of study were purchased from Charles River Laboratories (Germany).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cell-based ELISA: A549 cells were seeded at 2.9 × 104 cells/well in Dulbecco’s Modified Eagle Medium (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) in a flat-bottomed 96-well microtiter plate (Corning).	A549 suggested: None
   Assays were performed on HEK293T target …

   SciScore for 10.1101/2022.03.04.482636: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Euthanasia Agents: Blood samples were collected via the retro-orbital route under isoflurane anesthesia.
   Sex as a biological variable	Female BALB/c mice aged 8-10 weeks at the start of study were purchased from Charles River Laboratories (Germany).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cell-based ELISA: A549 cells were seeded at 2.9 × 104 cells/well in Dulbecco’s Modified Eagle Medium (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) in a flat-bottomed 96-well microtiter plate (Corning).	A549 suggested: None
   Assays were performed on HEK293T target cells stably expressing the human angiotensin-converting enzyme 2 (ACE2) and human transmembrane serine protease 2 (TMPRSS2) genes (VectorBuilder, Cat. CL0015).	HEK293T suggested: None
   After one hour incubation, the serum-particle mixture was inoculated onto HEK293T.ACE2.TMPRSS2 cells.	HEK293T.ACE2.TMPRSS2 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Female BALB/c mice aged 8-10 weeks at the start of study were purchased from Charles River Laboratories (Germany).	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   Data were analyzed using GraphPad Prism 9, using the “Specific binding with Hill slope” module to calculate the antibody binding affinity.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

   ---

   Results from scite Reference Check: We found no unreliable references.

   ---

   Read the original source
2. 

   Version published to 10.1101/2022.03.04.482636v1 on bioRxiv

   Mar 4, 2022