Detection of SARS-CoV-2 Omicron, Delta, Alpha and Gamma variants using a rapid antigen test
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Abstract
Throughout the coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have emerged with different infection and disease dynamics. Testing strategies, including clinical diagnosis, surveillance, and screening, have been deployed to help limit the spread of SARS-CoV-2 variants. Rapid antigen tests, in particular, have been approved for self-testing in many countries and governments are supporting their manufacturing and distribution. However, studies demonstrating the accuracy of rapid antigen tests in detecting SARS-CoV-2 variants, especially the new Omicron variant, are limited. We determined the analytical sensitivity of a CE-marked rapid antigen test against the Omicron, Delta, Alpha and Gamma variants. The rapid antigen test had the most sensitive limit of detection (10 plaque forming units [PFU]/mL) when tested with the Alpha and Gamma variants, followed by the Omicron (100 PFU/mL) and Delta (1,000 PFU/mL) variants. Given the increasing numbers of breakthrough infections and the need to surveil infectiousness, rapid antigen tests are effective public health tools to detect SARS-CoV-2 variants.
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SciScore for 10.1101/2022.01.27.22269299: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: The collection of isolates was approved by the Massachusetts General Hospital Institutional Review Board (approval number 2019P003305). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources In brief, the variants were isolated at the Ragon BSL3 by collection of the culture supernatant of Vero-E6 cells at 4-6 days post-infection with primary clinical specimens. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Image Analysis: The images of the rapid antigen tests were analyzed using Image J (NIH, Bethesda, MD) … SciScore for 10.1101/2022.01.27.22269299: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: The collection of isolates was approved by the Massachusetts General Hospital Institutional Review Board (approval number 2019P003305). Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources In brief, the variants were isolated at the Ragon BSL3 by collection of the culture supernatant of Vero-E6 cells at 4-6 days post-infection with primary clinical specimens. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Image Analysis: The images of the rapid antigen tests were analyzed using Image J (NIH, Bethesda, MD) for quantitative analysis of the results. Image Jsuggested: (ImageJ, RRID:SCR_003070)Statistics: GraphPad Prism 9.0 (San Diego, CA) was used to analyze and report the final test signal of the antigen tests. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
Results from scite Reference Check: We found no unreliable references.
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