1. SciScore for 10.1101/2022.01.13.22269213: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: Donors signed informed consent forms.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    KLRG1 Monoclonal Antibody (13F12F2), eBioscience,
    suggested: None
    Peptide stimulation was performed in the presence of costimulatory antibodies against CD28 and CD49d (BD Biosciences) and Brefeldin-A (10 μg/mL, Millipore Sigma).
    antibodies against CD28
    suggested: (Novus Cat# NB100-93558, RRID:AB_1236789)
    suggested: None
    Antibody staining panel setup: Purified CD19 (Biolegend), TCRVα7.2, CD160 and KLRG1 (R&D Systems) antibodies lacking carrier proteins (100 μg/antibody) were conjugated to DN3 MAXPAR chelating polymers loaded with heavy metal isotopes following the recommended labelling procedure (Fluidigm).
    suggested: None
    suggested: None
    suggested: None
    174Yb anti-PD anti-1/CD279
    suggested: (Fluidigm Cat# 3143013, RRID:AB_2661810)
    suggested: (Abcam Cat# ab120611, RRID:AB_11157298)
    Cells were washed, and each well was then stained with 100 μL of a unique double metal–labelled (Y89, Cd-106, Cd-110, Cd-112, Cd-116 and Cd-196) anti-CD45 antibody mix to further barcode the cells of individual donor.
    suggested: None
    All Antibodies were purchased from BD biosciences except towards Blimp-1 and IRF4 (Thermofischer) and IgM, CD71 and HLA-DR (Biolegend).
    suggested: None
    IgM , CD71
    suggested: None
    suggested: None
    Experimental Models: Cell Lines
    One Million cells per donor samples, healthy donor PBMCs and VeriCells were seeded in 96-well plate.
    suggested: None
    Software and Algorithms
    All participants had received both doses of SARS-CoV-2 vaccines (either BNT162 or mRNA-1273) according to the Norwegian National Vaccination Program.
    Norwegian National Vaccination Program
    suggested: None
    Data analysis was performed using CYTOGRAPHER® (ImmunoScape cloud based analytical software), custom R-scripts, GraphPad Prism (GraphPad Software) and FlowJo v10 software (BD Life Sciences).
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The Delta breakthrough samples were in addition stimulated with mutated Spike peptides (PepTivator
    suggested: None
    The rest of the following mAbs were directly purchased from Fluidigm: y89 anti-CD45, 106Cd anti-CD45, 110Cd anti-CD45
    suggested: (Fluidigm CyTOF, RRID:SCR_021055)
    Each sample was manually de-barcoded followed by gating on live CXCR5+CD4+ T cells for TFH analysis (CD45+ DNA+ cisplatin− CD3+ cells) after gating out residual antigen-presenting cells (HLA-DR+CD3- such as monocytes (CD14) and B cells (CD19) using FlowJo (Tree Star) software.
    suggested: (FlowJo, RRID:SCR_008520)
    A correlation matrix was calculated comparing phenotypic and serological marker variables in a pairwise fashion, using the corr.test function from the psych CRAN package; the corrplot package was subsequently used to graphically display the correlation matrix.
    suggested: (CRAN, RRID:SCR_003005)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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