1. SciScore for 10.1101/2022.01.12.22269048: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: Unrelated data from these healthy individuals have been presented elsewhere.13,14 All patients (CARDS and non-COVID-19) were sedated and unable to provide oral and written informed consent, which was therefore obtained from the next of kin.
    IRB: 5 The study was approved by the Regional Ethics Committee of Copenhagen (H-20023159/H-22011021/H-22009131) and the Knowledge Center for Data Review of Copenhagen (P-2020-399) and registered at ClinicalTrials.gov (NCT04354584).
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    19 IFN autoantibodies: IFN autoantibodies were measured in BALF by enzyme-linked immunosorbent assay (ELISA), as previously described for serum.20 Briefly, ELISA plates were coated with 1 μg/mL IFN-α (Miltenyi Biotec, Bergish Gladbach, Germany) and IFN-ω (ThermoFisher Scientific) overnight at 4°C followed by blocking in 5% skimmed milk.
    suggested: None
    Bound autoantibodies were detected with HRP-conjugated goat anti-human IgG, IgA, IgM (Fc specific)(Nordic-MUbio, Susteren, Netherlands) and HRP substrate KPL SureBlue (Seracare Life Sciences, Milford, MA, US).
    anti-human IgG
    suggested: None
    IgA, IgM
    suggested: None
    Experimental Models: Organisms/Strains
    All non-COVID-19 patients had ARDS and/or sepsis according to Berlin criteria11 and The Third International Consensus Definition for Sepsis and Septic Shock,12 respectively.
    Berlin criteria11
    suggested: None
    Software and Algorithms
    RNA yield and integrity were assessed using Qubit (ThemoFisher Scientific) and Bioanalyzer 2100 (Agilent, Santa Clara, CA, US) using the RNA nano chip.
    ThemoFisher Scientific
    suggested: None
    Sequence analysis: Reads from the two flow cells were de-multiplexed, pooled and mapped by Hisat2 v2.1.0 to the human reference genome GRCh38 obtained from Ensembl.
    suggested: (HISAT2, RRID:SCR_015530)
    suggested: (Ensembl, RRID:SCR_002344)
    Gene expression profiles were obtained from the sorted bam files using featureCounts v2.0.0 and the Ensembl annotation GRCh38.103.
    suggested: (featureCounts, RRID:SCR_012919)
    Differential analysis was performed in R, as described previously.15 Briefly, we used the Limma-voom and DeSeq2 package for pre-processing and principal component (PC) analysis, respectively, and figures were produced using the ggplot2 package.
    suggested: (DESeq2, RRID:SCR_015687)
    suggested: (ggplot2, RRID:SCR_014601)
    Gene ontology (GO) analysis was performed using Goseq on up and downregulated genes separately for each contrast.
    suggested: (Goseq, RRID:SCR_017052)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    NCT04354584CompletedCompartmental Inflammation in Mechanically Ventilated Patien…

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

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