A synthetic bispecific antibody capable of neutralizing SARS-CoV-2 Delta and Omicron

  1. Tom Z. Yuan
  2. Carolina Lucas
  3. Valter S. Monteiro
  4. Akiko Iwasaki
  5. Marisa L. Yang
  6. Hector F. Nepita
  7. Ana G. Lujan Hernandez
  8. Joseph M. Taft
  9. Lester Frei
  10. Sai T. Reddy
  11. Cédric R. Weber
  12. Kevin P. Malobisky
  13. Rodrigo Mesquita
  14. Aaron K. Sato

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Abstract

Bispecific antibodies have emerged as a promising strategy for curtailing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immune escape. This brief report highlights RBT-0813 (also known as TB493-04), a synthetic, humanized, receptor-binding domain (RBD)-targeted bispecific antibody that retains picomolar affinity to the Spike (S) trimers of all major variants of concern and neutralizes both SARS-CoV-2 Delta and Omicron in vitro .

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  1. ScreenIT

    SciScore for 10.1101/2022.01.04.474803: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line AuthenticationContamination: The cell line has been tested negative for contamination with mycoplasma.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    The data was analyzed using FlowJo V10.4.2.
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)
    Analyses of plaque counts were done using GraphPad Prism software, version 8.4.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your code.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1101/2022.01.04.474803v1 on bioRxiv