Preserved recognition of Omicron spike following COVID-19 messenger RNA vaccination in pregnancy

  1. Yannic C. Bartsch
  2. Caroline Atyeo
  3. Jaewon Kang
  4. Yongfei Cai
  5. Bing Chen
  6. Kathryn J. Gray
  7. Andrea G. Edlow
  8. Galit Alter

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.ajog.2022.04.009
  2. ScreenIT

    SciScore for 10.1101/2022.01.01.22268615: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: All participants gave informed consent prior to enrollment and this study was approved by the Mass General Brigham Institutional Review Board (IRB; protocol #2020P003538).
    IRB: All participants gave informed consent prior to enrollment and this study was approved by the Mass General Brigham Institutional Review Board (IRB; protocol #2020P003538).
    Sex as a biological variableStudy Population: To compare vaccine-induced antibody responses to SARS-CoV-2 VOCs in pregnant individuals, samples from 10 pregnant patients receiving the full two dose regimen BNT162b2 (Pfizer) andr from 10 pregnant patients receiving the full two dose regimen mRNA-1273 (Moderna) were obtained 2-4 weeks after the second dose.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    IgG subclass, isotype and FcγR binding: Antigen-specific antibody subclass and isotypes, and FcγR binding was analyzed by Luminex multiplexing.
    IgG subclass,
    suggested: None
    Antigen-specific
    suggested: None
    Unbound antibodies were washed away and subclasses, isotypes were detected with a respective PE-conjugated antibody (anti-human IgG1, IgG2, IgG3, IgG4, IgM or IgA1 all SouthernBiotech, AL, USA) at a 1:100 dilution.
    anti-human IgG1
    suggested: None
    IgG2
    suggested: None
    IgG3, IgG4
    suggested: None
    IgA1
    suggested: (LSBio (LifeSpan Cat# LS-C68444-100, RRID:AB_1649687)
    Experimental Models: Cell Lines
    SentencesResources
    Stabilized (hexa-pro) Spike of D614G (“wildtype” variant) or VOCs was produced in HEK293 cells.
    HEK293
    suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a protocol registration statement.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.01.01.22268615v1 on medRxiv