Inactivation of SARS-CoV-2 and influenza A virus by spraying hypochlorous acid solution and hydrogen peroxide solution in the form of Dry Fog

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1. 

   ScreenIT

   Dec 17, 2021

   SciScore for 10.1101/2021.12.13.472413: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cells, viruses, and preparation of dried virus samples: VeroE6/TMPRESS2 cells (JCRB1819) [11] were maintained in Dulbecco’s modified Eagle medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Merck, Kenilworth, New Jersey, USA) and 1 mg/ml of G418 (Sigma-Aldrich) (complete DMEM), while MDCK cells were maintained in Eagle’s minimum essential medium (MEM) (MEM1, Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) …

   SciScore for 10.1101/2021.12.13.472413: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cells, viruses, and preparation of dried virus samples: VeroE6/TMPRESS2 cells (JCRB1819) [11] were maintained in Dulbecco’s modified Eagle medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Merck, Kenilworth, New Jersey, USA) and 1 mg/ml of G418 (Sigma-Aldrich) (complete DMEM), while MDCK cells were maintained in Eagle’s minimum essential medium (MEM) (MEM1, Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) supplemented with 10% FBS in a CO2 incubator.	MDCK suggested: None
   The SARS-CoV-2 Wuhan strain, SARS-CoV-2/Hu/DP/Kng/19-020 was propagated by infecting VeroE6/TMPRESS2 cells and cultured in FBS-free DMEM supplemented with 1 mg/ml of G418 for 24 hours.	VeroE6/TMPRESS2 suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistical analysis: Statistical analyses were performed using the standard function of GraphPad Prism 8 software (GraphPad Software, San Diego, California) with a 2-way ANOVA, unpaired t-test, a non-linear regression (curve fit) analysis, or simple linear regression analysis.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   There are a number of limitations that need to be addressed. Only the SARS-CoV-2 Wuhan strain and influenza A virus H1N1 strain were tested in the present study. SARS-CoV-2 variants of concern, including the delta variant, are continually emerging. Furthermore, there is a wide variety of influenza A viral strains. Inactivation experiments were not performed on these viral strains in the present study. However, a previous study suggested that hypochlorous acid attacks multiple components of microorganisms, including the plasma membrane and nucleic acids, as its germicidal effect [10]. Although the structures of viral proteins due to genetic mutations may differ between the Wuhan strain and other SARS-CoV-2 variants, there may be a commonality in the basic structures and components of virions, such as the lipid bilayer (envelope) and structural and non-structural proteins. Therefore, Dry Fog spraying is expected to act effectively not only against the Wuhan strain and H1N1 strain tested in the present study, but also against other virus strains. As another limitation, we only examined the effects of Dry Fog spraying on viruses dried on the surfaces of plastic microplates. Further studies are warranted to investigate its effects on viruses adhering to the surfaces of other materials. Furthermore, the mechanisms by which Dry Fog spraying affects the infectivities of viruses in a space need to be elucidated. However, difficulties may be associated with establishing an experimental...

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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2. 

   Version published to 10.1101/2021.12.13.472413v1 on bioRxiv

   Dec 13, 2021