Peptide–Antibody Fusions Engineered by Phage Display Exhibit an Ultrapotent and Broad Neutralization of SARS-CoV-2 Variants
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SciScore for 10.1101/2021.11.29.470362: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization Oligonucleotide-directed mutagenesis was performed to introduce randomized sequences fused to the gene-8 major coat protein of M-13 bacteriophage as described23. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Plates were washed, incubated with anti-M13-HRP antibody (Sino Biological, catalog number 11973-MM05T-H) and developed with TMB substrate (Mandel, catalog number KP-50-76-03). anti-M13-HRPsuggested: NoneIn addition, we monitored infection using an ELISA-based method20 with the anti-S-protein antibody CR302225, followed by incubation with … SciScore for 10.1101/2021.11.29.470362: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization Oligonucleotide-directed mutagenesis was performed to introduce randomized sequences fused to the gene-8 major coat protein of M-13 bacteriophage as described23. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Plates were washed, incubated with anti-M13-HRP antibody (Sino Biological, catalog number 11973-MM05T-H) and developed with TMB substrate (Mandel, catalog number KP-50-76-03). anti-M13-HRPsuggested: NoneIn addition, we monitored infection using an ELISA-based method20 with the anti-S-protein antibody CR302225, followed by incubation with HRP-conjugated goat anti-Fc antibody (Invitrogen, catalog number A18817). anti-S-proteinsuggested: (PhosphoSolutions Cat# CoV2-5G8, RRID:AB_2868396)anti-Fcsuggested: NoneExperimental Models: Cell Lines Sentences Resources The IgG-virus mixture was transferred to 96-well tissue culture plates containing sub-confluent monolayers of Vero E6 cells in duplicate and incubated at 37 °C and 5% CO2. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources Sequence alignment and analysis: Sequences of binding peptides were imported into Geneious R9 software (Biomatters Ltd.). Geneioussuggested: (Geneious, RRID:SCR_010519)Peptides were sorted based on the library from which they originated and aligned separately using the MUSCLE algorithm. MUSCLEsuggested: (MUSCLE, RRID:SCR_011812)All data were analyzed using Prism software (GraphPad Prism 8.0). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)To build the model of two RGN10933 Fabs bound to the S-protein, the model of RGN10933 and RGN10987 bound to the RBD (PDB entry 6XDG) was imported into PyMol along with the data from PDB entry 7KMK. PyMolsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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