Potent antibody immunity to SARS‐CoV‐2 variants elicited by a third dose of inactivated vaccine

  1. Bin Ju
  2. Bing Zhou
  3. Shuo Song
  4. Qing Fan
  5. Xiangyang Ge
  6. Haiyan Wang
  7. Lin Cheng
  8. Huimin Guo
  9. Dan Shu
  10. Lei Liu
  11. Zheng Zhang

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Abstract

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  1. Version published to 10.1002/ctm2.732
  2. ScreenIT

    SciScore for 10.1101/2021.11.10.468037: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Study approval and blood samples: This study was approved by the Ethics Committee of Shenzhen Third People’s Hospital, China (approval number: 2020-030).
    Consent: All participants had provided written informed consent for sample collection and subsequent analysis.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    The plates were washed, and HRP-conjugated goat anti-human IgG antibodies (ZSGB-BIO) were added and then incubated at 37 °C for 30 mins.
    anti-human IgG
    suggested: None
    Flow cytometric analysis of RBD-specific memory B cells: Thawed PBMCs were stained with an antibody cocktail consisting of CD19- PE-Cy7, CD3-Pacific Blue, CD8-Pacific Blue, CD14-Pacific Blue, CD27-APC-H7, and IgG-FITC (all from BD Biosciences) to gate IgG+ memory B cells.
    CD27-APC-H7
    suggested: None
    IgG-FITC
    suggested: None
    Two anti-His secondary antibodies separately labeled with APC and PE (Abcam) were both used to recognize the RBD bait and exclude nonspecific staining.
    anti-His
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    SARS-CoV-2 pseudovirus-based neutralizing assay: SARS-CoV-2 pseudovirus was generated by cotransfection of HEK-293T cells with SARS-CoV-2 spike-expressing plasmid and an env-deficient HIV-1 backbone vector (pNL4-3.Luc.R-E-).
    HEK-293T
    suggested: None
    HEK-293T-hACE2 cells were subsequently added to the plates.
    HEK-293T-hACE2
    suggested: RRID:CVCL_A7UK)
    Recombinant DNA
    SentencesResources
    SARS-CoV-2 pseudovirus-based neutralizing assay: SARS-CoV-2 pseudovirus was generated by cotransfection of HEK-293T cells with SARS-CoV-2 spike-expressing plasmid and an env-deficient HIV-1 backbone vector (pNL4-3.Luc.R-E-).
    HIV-1
    suggested: RRID:Addgene_115809)
    pNL4-3.Luc.R-E-
    suggested: None
    Software and Algorithms
    SentencesResources
    The 50% inhibitory dilution (ID50) was calculated using GraphPad Prism 8.0 software by log (inhibitor) vs. normalized response - Variable slope (four parameters) model.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Flow cytometric data were acquired on an Aria II flow cytometer (BD Biosciences) and analyzed using FlowJo software (TreeStar).
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.11.10.468037 on bioRxiv