Novel Investigation of SARS-CoV-2 in COVID-19 Survivors’ Semen in Surabaya, Indonesia
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Abstract
The emergence and the widespread of Coronavirus disease 2019 (COVID-19) demands an accurate detection method to establish a diagnosis. Real-time polymerase chain reaction (real-time PCR) is accounted for the perfect point of reference in detecting this virus. The notion that this virus also invades the male reproductive tract requires further investigation to prove the presence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in the semen. This investigation was designed to detect SARS-CoV-2 in COVID-19 survivors’ semen. This study design was a cross-sectional examination and conducted between November 2020 and March 2021 in the Andrology Unit of Dr. Soetomo General Hospital and Professor Nidom Foundation, both located in the City of Surabaya, Indonesia. The sample was 34 male participants aged above 18 years old and had been confirmed COVID-19 by nasopharyngeal swab PCR test. Part of the semen was taken for real-time PCR testing with the QuantStudio 5 Applied Biosystem (AB) PCR machine and the kits utilized were the STANDARD M nCOV Real-Time Detection Kit and mBioCov-19 RT-PCR Kit. Furthermore, the mean of participants’ ages was 35.74 years old with 25% of them had had a history of primary infertility and 21.8% of secondary infertility. From the real-time PCR COVID-19 of the semen examination, this investigation found that 27 participants had been negatives (74.4%), six inconclusive (17.6%), and one positive (3%) of SARS CoV-2. In summary, SARS-CoV-2 could be found in the semen of COVID-19 survivors. This should be a concern for the potential impact of COVID-19 in male fertility and the possibility of transmission reproductively.
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SciScore for 10.1101/2021.10.08.21264593: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable A total of 34 male participants were registered as research subjects. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Participants were then instructed to abstinence 2-7 days and wash their hands before doing masturbation for the semen collection. 2.3. Real-Time PCR: The examination was implemented using RT-PCR with QuantStudio 5 Applied Biosystem (AB) PCR machine, the kit applied was STANDARD M nCOV Real-Time Detection Kit (Lot #MNCO0120026) and MBioCoV-19 RT-PCR Kit (Lot # 6900820) carried out at the Research Group of Coronavirus and Vaccine … SciScore for 10.1101/2021.10.08.21264593: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable A total of 34 male participants were registered as research subjects. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Participants were then instructed to abstinence 2-7 days and wash their hands before doing masturbation for the semen collection. 2.3. Real-Time PCR: The examination was implemented using RT-PCR with QuantStudio 5 Applied Biosystem (AB) PCR machine, the kit applied was STANDARD M nCOV Real-Time Detection Kit (Lot #MNCO0120026) and MBioCoV-19 RT-PCR Kit (Lot # 6900820) carried out at the Research Group of Coronavirus and Vaccine Formulation, at Professor Nidom Foundation, Surabaya, Indonesia. ABsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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