Diversity of SARS-CoV-2 genome among various strains identified in Lucknow, Uttar Pradesh
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a significant challenge worldwide. Rapid genome sequencing of SARS-CoV-2 is going on across the globe to detect mutations and genomic modifications in SARS-CoV-2. In this study, we have sequenced twenty-three SARS-CoV-2 positive samples collected during the first pandemic from the state of Uttar Pradesh, India. We observed a range of already reported mutations (2-22), including; D614G, L452R, Q613H, Q677H, T1027I in the S gene; S194L in the N gene; Q57H, L106F, T175I in the ORF3. Few unreported mutations such as P309S in the ORF1ab gene; T379I in the N gene; and L52F, V77I in the ORF3a gene were also detected. Phylogenetic genome analysis showed similarity with other SARS-CoV-2 viruses reported from Uttar Pradesh. The observed mutations may be associated with SARS-CoV-2 virus pathogenicity or disease severity.
Article activity feed
-
-
SciScore for 10.1101/2021.10.05.463185: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: This study protocol was approved by Institutional Human Ethics Committee SGPGIMS, Lucknow (Ref N. 111 PGI/BE/327/2020) Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources MAFFT was used to align the whole sequences of the 24 genomes to SARS-CoV-2 reference genome (MN908947.3)[17], [18]. MAFFTsuggested: (MAFFT, RRID:SCR_011811)SnpEff v4.3 was used to identify the SNPs and changes in the amino acid produced by the gene in the genome[19]. SnpEffsuggested: (SnpEff, RRID:SCR_005191)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An …SciScore for 10.1101/2021.10.05.463185: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: This study protocol was approved by Institutional Human Ethics Committee SGPGIMS, Lucknow (Ref N. 111 PGI/BE/327/2020) Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources MAFFT was used to align the whole sequences of the 24 genomes to SARS-CoV-2 reference genome (MN908947.3)[17], [18]. MAFFTsuggested: (MAFFT, RRID:SCR_011811)SnpEff v4.3 was used to identify the SNPs and changes in the amino acid produced by the gene in the genome[19]. SnpEffsuggested: (SnpEff, RRID:SCR_005191)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
-