Durability of immune responses to the BNT162b2 mRNA vaccine

Article activity feed

1. Version published to 10.1016/j.medj.2021.12.005

   Jan 1, 2022
2. 

   ScreenIT

   Oct 4, 2021

   SciScore for 10.1101/2021.09.30.462488: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Human subjects and experimentation: Fifty-six healthy volunteers were recruited for the study at the Stanford University under informed consent. IRB: The study was approved by Stanford University under Institutional Review Board protocols 8269 and 60171.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Cells were incubated with an anti-SARS-CoV spike primary antibody directly conjugated with alexaflour-647 (CR3022-AF647) for up to 4 hours at room temperature.	anti-SARS-CoV spike suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources

   SciScore for 10.1101/2021.09.30.462488: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Human subjects and experimentation: Fifty-six healthy volunteers were recruited for the study at the Stanford University under informed consent. IRB: The study was approved by Stanford University under Institutional Review Board protocols 8269 and 60171.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Cells were incubated with an anti-SARS-CoV spike primary antibody directly conjugated with alexaflour-647 (CR3022-AF647) for up to 4 hours at room temperature.	anti-SARS-CoV spike suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   The hCoV-19/Japan/TY7-503/2021 (P.1) was provided by BEI resources and propagated in Vero-TMPRSS2 cells.	Vero-TMPRSS2 suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)
   The antibody-virus mixture was then added to VeroE6-TMPRSS2 cells and incubated at 37° C for 1 hour.	VeroE6-TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   hCoV-19/USA/CA-Stanford-15_S02/2021 (herein referred to as the B.1.617.1 variant) was derived from a mid-turbinate nasal swab collected from a Stanford Healthcare patient in March 2021 and isolated as previously described16.	Stanford Healthcare suggested: None
   The FRNT-50 titers were interpolated using a 4-parameter nonlinear regression in GraphPad Prism 8.4.3.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   All flow cytometry data were analysed using Flowjo software v.10 (TreeStar).	Flowjo suggested: (FlowJo, RRID:SCR_008520)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT04828603	Recruiting	Understanding Allergies and Sensitizations in Healthy and Al…
   NCT04664309	Recruiting	Understanding Immunity to the COVID-19 Vaccines

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.09.30.462488 on bioRxiv

   Sep 30, 2021